Neil Gudmestad - NDSU Publications

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Browsing Neil Gudmestad - NDSU Publications by browse.metadata.program "Plant Sciences"

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    Blizzard Watch : Plant Sciences : 2017
    (North Dakota State University, 2018) North Dakota State University. Department of Plant Sciences
    Newsletter for the Department of Plant Sciences.
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    Glycoalkaloid Profiling of Potato Genotypes from the North Dakota State University Potato Breeding Program
    (North Dakota State University, 2013) Roman Martinez, Irene
    Gylcoalkaloids (GA) are plant secondary metabolites that offer pests and disease resistance. Studies show correlation between GA content and CPB resistance. In this study, CPB resistance was assessed in a field trial at Grand Forks, ND, during 2012 for twenty-four genotypes from the NDSU Potato Breeding Program. Two treatments were applied, a block treated with imidacloprid (Admire®), and an untreated block. The treated block showed decreased CPB damage. Presence of aglycons (non-sugar moiety of GAs) was assessed by gas chromatography in foliar and tuber tissue. Distribution of GAs in the tuber was assessed to determine variation in tuber sections and whole tuber. Potato genotypes should be developed with tuber GAs levels below 20 mg/100 g fresh weight (FW) to ensure safety for human consumption. Focus should be on GAs that are only synthesized in the tuber, which will provide pests and disease resistance, while maintaining adequate yields and decreased inputs.
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    Marker Assisted Selection Increases the Efficiency of Breeding for Potato Virus Y Resistance in Potato
    (North Dakota State University, 2014) Harchenko, Whitney Ann
    Potato Virus Y (PVY) is an important virus of potato due to the non-persistent mode of transmission by aphids causing yield losses. Genetic resistance is the recommended control since insecticides cannot adequately control the spread of PVY by aphids. The gene Ryadg from S. tuberosum ssp. andigena provides resistance to all strains of PVY. This gene has genetically been mapped to chromosome XI, and linked polymerase chain reaction (PCR) based DNA markers have been identified. This study identified PVY resistant progeny by the use of the molecular sequence-characterized amplified region (SCAR) marker RYSC3. The RYSC3 marker allowed a simple and fast approach to determine if the Ryadg gene was present in the seedling family populations evaluated. The RYSC3 marker identified 16 families with progeny segregating for the Ryadg gene. Progeny segregated 1:1 for PVY resistance, fitting the model simplex (Ryryryry) for the Ryadg allele.