Many studies have been performed on exploring the effects of radio frequency energy on biological function in vivo. In particular, gene expression results have been inconclusive due, in part, to a lack of a standardized experimental procedure. This thesis describes a new far field RF exposure system for unrestrained murine models that reduces experimental error and a 31 day experiment using mice in vivo. The experiment uses whole body exposure to continuous RF energy at 2.45 GHz on unrestrained, in vivo mice. Using RNA-seq to analyze the entire murine genome, the data is statistically analyzed using combinations and empirical p-values. The analyzed data’s genome are explored using Ingenuity Pathway Analysis to locate gene functional groups within heart tissues. Results show an intriguing finding of a discrete/continuous system due to radio frequency energy, along with genes alteration found in heart functional groups.