Chrononutrition, or the circadian timing of food intake, has garnered attention as a topic of study due to its associations with health (e.g., weight gain); however, a valid assessment of chrononutrition in daily life has not yet been developed. The present study therefore aimed to develop and validate both a diary and questionnaire version of the Chrononutrition Profile which assess 6 components of chrononutrition that have been associated with poor health (breakfast skipping, night eating, eating window, evening latency, largest meal, and evening eating). The measure demonstrated preliminary evidence of test-retest reliability and convergent validity, though concurrent validity was not interpretable. Based on analyses, the final diary and questionnaire versions of the CP assess 5 components of chrononutrition: breakfast skipping, night eating, eating window, evening latency, and evening eating. This measure offers health care professionals, researchers, and stakeholders a cost-effective method of evaluating chrononutrition and identifying targets for health improvement.