Carbon Nanotubes-Based Lateral Flow Biosensor for Sensitive and Rapid Detection of DNA Sequence

Abstract

Sensitive detection for DNA has drawn great attention in plenty of different areas such as genetics therapy and basic discovery research. Recently, nucleic acid lateral flow biosensor (NALFB) has gained considerable attention for DNA analysis. Compared with the traditional immunoassays, NALFB has these advantages: short assay time and a low cost. In this thesis, we report a carbon nanotubes (CNTs)-based NALFB for rapid and sensitive detection of DNA. Amine-modified DNA detection probe was covalently immobilized on the CNTs via diimide-activated amidation between the carboxyl groups on the CNTs surface and amine groups on the detection DNA probes. Sandwich-type DNA hybridization reactions were performed and the captured MWCNTs on test zone and control zone produced the characteristic black bands. Based on the catalytic property of CNTs to enhance the Chemiluminescence intensity of the reaction between hydrogen peroxide and Lumigen APS-5, a rapid detection of DNA sequence with high sensitivity is achieved.