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Subject: dielectrophoresis

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    Label-Free CD8+ T-cell Purification and Electroporation in Relation to CAR T-cell Therapy
    (North Dakota State University, 2020) Ringwelski, Beth Anne
    Immunotherapy is becoming recognized as a superior treatment for cancer. In recent years, chimeric antigen receptor (CAR) therapy is among the immunotherapies that has had growing success rates. CAR T-cell therapy takes patient’s T-cells and encodes them with a CAR expressing gene, which can then target their cancer cells. However, there are some dangers associated with this therapy. If a cancer cell is mistakenly transfected with the CAR molecule, it can become resistant to the therapy. Using the electric properties of the cells, we have created a technique that can purify the T-cells from the remaining cancer cells using microfluidics and dielectrophoresis (DEP). Then, to further improve the therapy, the sample is electroporated following being patterned using DEP forces, which transfects the cells without using viral vectors and provides longer CD19 expression.
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    Label Free Micro-RNA Biomarker Detection in Serum Samples for Potential Diagnosis Application at Point-of-Care Settings
    (North Dakota State University, 2020) Pokharel, Rounak
    The number of new cancer cases is projected to rise to 23.6 million by 2030 according to the National Cancer Institute. Obesity & cardiovascular diseases are among the leading causes of death worldwide according to recent reports. Biomarkers— any molecules found within a human body that can be used to monitor an individual's health — have been shown to play a significant role in the detection of cancer, obesity, and cardiovascular diseases. Recent studies have shown that in the diagnosis and screening of various human diseases, including cancer, obesity and cardiovascular diseases, circulating microRNAs (miRNAs) are important biomarkers. A crucial roadblock to using microRNA in screening applications is the lack of effective and low-cost microRNA detection. To address this issue, in this study, we have developed a viable method that combines the dielectrophoresis and electrical impedance. Results show this approach can measure very small concentrations of label-free microRNAs (1pM).