Prebiotic carbohydrates are an important component of healthy diet. The objectives of this study were (1) to compare the concentrations of prebiotic carbohydrates in different lentil genotypes and growing locations and (2) to compare the concentrations of prebiotic carbohydrates in lentil under various processing and preparation procedures. Mean concentrations of prebiotics in lentil genotypes were as follows: raffinose-family oligosaccharides (RFO) (raffinose + stachyose, 2509 mg; verbascose, 1562 mg), fructo-oligosaccharides (nystose, 62 mg), sugar alcohols (sorbitol, 1220 mg; mannitol, 203 mg), and resistant starch (RS), 7.5 g 100 g-1. Modest RFO concentration reductions were observed with cooking, cooling, and reheating. Mean RS concentration in raw, cooked, cooled, and reheated lentil were 3.0, 3.0, 5.1, and 5.1 g/100g respectively, clearly demonstrating cooling-induced synthesis of RS. Study results suggest that lentil contains nutritionally significant concentrations of prebiotics and that those concentrations may be enhanced through breeding, locational sourcing, and cooking and preparation procedures.