No experimental research has examined the effect of drinking multiple boluses of pickle juice (PJ) on the same day, nor has its ingestion been examined during exercise. Additionally there are fears that PJ supplementation can cause hyperkalemia. We determined the effect of ingesting single or multiple boluses of PJ on plasma variables before, during, and after exercise. On three days, subjects ingested 0, 1, or 2 boluses of PJ and biked vigorously for 60 minutes. Blood samples were collected pre-ingestion and 30, 65, 95, and 125 minutes post ingestion. The number of PJ boluses consumed did not affect [Na+]p, [K+]p, OSMp, or changes in plasma volume over time. Ingesting up to 2 boluses of PJ and resuming exercise causes negligible changes in blood variables and will not increase [Na+]p or cause hyperkalemia.