The objective of this study was to investigate the effects of electrical stimulation on the cellular chemistry and proliferation of C2C12 muscle cell line. The cells were cultured under the condition of AC electrical stimulation using interdigitated electrode arrays. This research was conducted by applying electrical signals for up to 24 hours to C2C12 muscle cells. After 24 hours, the electrical impedance, cell morphology, proliferation and viability were recorded. The results demonstrated that electrical stimulation have a positive impact on the cell growth of C2C12. These results obtained would aid in future study on improving the techniques of culturing C2C12 muscle cells and also in the field of electrical conductivity in cell proliferation. Lastly, the results obtained also would provide essential information to the studies related to gene and protein expression of cellular and subcellular components after being exposed to electrical stimulation.