There is currently no standard method to measure the incidence and prevalence of musculoskeletal injury among musicians. The purpose of this study was to evaluate the validity and reliability for the Musculoskeletal Pain Questionnaire for Musicians (MPQM) and the Musculoskeletal Pain Intensity and Interference Questionnaire for Musicians (MPIIQM) in collegiate musicians. Participants completed a packet containing the MPQM and MPIIQM, with two baseline instruments (SF-36 and QuickDASH). Convergent validity was evaluated using a correlation between the scores of the baseline instruments and the MPQM and MPIIQM. Reliability was evaluated using the split-half method and Cronbach’s alpha. The readability was evaluated with the Flesch-Kincaid score. The MPQM and MPIIQM showed weak correlations to the SF-36 and moderate correlations to the QuickDASH and the sports/performing arts optional module. Both showed strong reliability and good readability. Both should be used with caution in future research.