Kinesio® Tape has the potential to optimize the treatment of subacromial impingement syndrome. This research project investigated the effect Kinesio® Tape has on patient-reported outcome measures and acromiohumeral distance in patients with subacromial impingement syndrome. Twenty volunteers exhibiting subacromial impingement syndrome symptoms were divided into two groups, one receiving Kinesio® Tape inhibition technique of the supraspinatus and deltoid muscles and the other receiving a sham Kinesio® Tape. Patient-reported SPADI scores and acromiohumeral distance measured by diagnostic ultrasound were recorded at 24- and 48-hour intervals. SPADI scores of both groups were statistically significantly lower at the 48-hour interval. No statistically significant change in acromiohumeral distance was found at any interval. Therefore, Kinesio® Tape on the supraspinatus and deltoid muscles alleviated symptoms related to subacromial impingement syndrome as reported by patient-outcome data but did not alter the subacromial space according to diagnostic ultrasound scanning.