| dc.contributor.author | Petersen, Morgan L. | |
| dc.description.abstract | Biofilm formation may increase survival and persistence of Escherichia coli in the highly variable conditions of soil environments, though it remains unknown the extent variation in biofilm formation affects survival. We asked what genetic traits influence biofilm formation in phylogroup D E. coli isolates from surface soils, and are they associated with the soil environment? Biofilm density was analyzed and compared with soil environment characteristics. Isolates produced more biofilm per unit growth at 15°C than 37°C. Biofilm formation was greater in soil isolates than fecal isolates and in soils with moisture and higher calcium and pH levels. A GWAS analysis found variants involved in cell envelope formation and structure were associated with biofilm formed at 37°C, and stress response and iron acquisition variants were associated with biofilm formed at 15°C. Motility variants were associated with a negative effect on biofilm formed and adhesion variants associated with a positive effect. | en_US |
| dc.publisher | North Dakota State University | en_US |
| dc.rights | NDSU policy 190.6.2 | |
| dc.title | Biofilm Formation of Escherichia coli from Surface Soils is Influenced by Variation in Cell Envelope, Iron Metabolism, and Attachment Factor Genes | en_US |
| dc.type | Thesis | en_US |
| dc.date.accessioned | 2018-09-26T16:55:15Z | |
| dc.date.available | 2018-09-26T16:55:15Z | |
| dc.date.issued | 2018 | en_US |
| dc.identifier.uri | https://hdl.handle.net/10365/28869 | |
| dc.subject.lcsh | Escherichia coli -- Environmental aspects. | |
| dc.subject.lcsh | Escherichia coli -- Genetics. | |
| dc.subject.lcsh | Biofilms. | |
| dc.subject.lcsh | Soils -- Environmental aspects. | |
| dc.description.sponsorship | National Science Foundation (NSF) award no. DEB-1453397 to P.W.B. | en_US |
| dc.description.sponsorship | ND-EPSCoR | en_US |
| dc.rights.uri | https://www.ndsu.edu/fileadmin/policy/190.pdf | |
| ndsu.degree | Master of Science (MS) | en_US |
| ndsu.college | Agriculture, Food Systems and Natural Resources | en_US |
| ndsu.department | Microbiological Sciences | en_US |
| ndsu.program | Microbiology | en_US |
| ndsu.advisor | Bergholz, Peter | |
