Trait mindfulness has been found to be a beneficial characteristic of athletes. However, the research on mindfulness interventions has been limited, poorly described, and poorly designed. The current study sought to determine whether a brief mindfulness intervention improves distress tolerance among athletes. In addition, this experiment tested the impact of cultivating mindfulness on psychological variables that may be important for sport such as anxiety, happiness and capacity for stress. Athletes were randomly assigned to one of the three intervention conditions (brief mindfulness, sham mindfulness and no-intervention control). All participants completed distress tolerance measures, a motor performance measure under distressing conditions, and self-reported psychological measures. These measures were administered at pre- and post-intervention. Results indicated that the brief mindfulness intervention did not result in significant improvements in the primary outcome variables, in comparison to the sham mindfulness and no-intervention control groups. Strengths and limitations of the study, as well as future directions are provided.