Bio-based flax fiber polymer composites (FFPC) have the potential to replace metals and synthetic fibers in certain applications due to their unique mechanical properties. However, the long term reliability of FFPC needs to be better understood. In this study, the fatigue limit was evaluated using mathematical, thermographic, and energy-based approaches. Each approach determined fatigue limits around 45% load of ultimate tensile strength at a loading frequency of 5 Hz. Thermographic and energy-based approaches were also implemented at different loading frequencies (5, 7, 10, and 15 Hz) to define the effect of loading frequency on the fatigue life. Fatigue limit was found to decrease slowly with increasing loading frequency. Moreover, two forms of damage energy (thermal and micro-mechanical) during cyclic loading was separated using an experimental approach to pinpoint the main responsible damage energy for decreasing fatigue limit with increasing loading frequency.