Interaction of flow structures from a three-cylinder system is complex and important for fundamental and engineering applications. In this study, experiments using hotwire, 2D PIV, and Tomography are to be conducted to characterize the fluid flow at various Re number and rotation speeds. The Reynolds number considered based on the diameter of the single-cylinder ranges from 37 to 1700. The peaks in the frequency spectrum obtained from the hotwire study show a unique relation of Strouhal number as a function of static incident angle, RPM, and Reynolds number. From the 2D PIV and 3D tomography experiment, vorticity and velocity results characterize the interaction of wake flow from individual cylinders and as a function of the rotational speeds. Besides, the Standard deviation map shows the turbulence intensity variation at the various static and rotating conditions. The obtained results at static conditions are found to be consistent with the previous computational study.