Sugar beet pulp (SBP), the residue remaining after sucrose extraction, is currently sold as an animal feed. Humans cannot digest the cellulose in the pulp unlike ruminant animals. The pulp is primarily comprised of cellulose, hemicellulose, and pectin which can be hydrolyzed with commercial enzymes into fermentable sugars such as, glucose, arabinose, galacturonic acid, xylose, and galactose. These sugars can be fermented to produce ethanol. This research tested the variation of several enzymes, enzyme loading rates, solids loading rates, and fermenting organisms to increase ethanol yields from sugar beet pulp. Several commercial enzymes (cellulases, hemicellulases, pectinases, and proteases) were tested to determine impact on SBP hydrolysis. Two commercial enzyme preparations (Viscozyme and Pectinex) were compared. Viscozyme produced the highest sugar yields because of increased cellulose hydrolysis, while Pectinex showed less cellulase activity. All enzyme treatments resulted in similar hemicellulose and pectin hydrolysis. Pretreatment with proteases reduced sugar yields from hydrolysis by 10-30% compared to hydrolysis without pretreatment. Escherichia coli K011, a genetically modified organism (GMO), and Saccharomyces cerevisiae were used to ferment SBP hydrolyzate to increase ethanol yields (g EtOH/g SBP) and concentrations (g/L). In the "Parallel" fermentation, pectinase was used to solubilize pectin and hemicellulose. After separation, the liquid stream was fermented with E. coli K011 and the high-cellulose solid fraction was fermented using S. cerevisiae and additional cellulase enzymes (Celluclast and Novozyme 188). The "Parallel" method initially produced under 0.15 g EtOH/g SBP but was improved with pH regulation to yield 0.23 g EtOH/g SBP. The separation method limited ethanol production. The ethanol yields from three additional fermentation methods ("E. coli K011 Only", "Serial", and "Reverse Serial") were compared. The "E. coli K011 Only" method was the baseline fermentation for comparison of the remaining three fermentation methods. SBP was hydrolyzed with pectinase, cellulase, and cellobiase before fermentation with E. coli K011 to yield 0.192 g ethanol/ g SBP. The total hydrolysis of the SBP limited ethanol production. The "Serial" fermentation began by solubilizing pectin and hemicellulose with pectinases. All of the flask contents were fermented with E. coli K011. The remaining cellulose-rich SBP was then hydrolyzed with cellulases and fermented by S. cerevisiae. Initial ethanol yields were under 0.15 g EtOH/g SBP but improved to 0.238 g EtOH/g SBP. Acetic acid concentrations limited ethanol production by S. cerevisiae. The "Reverse Serial" simultaneous saccharification and fermentation (SSF) started with pectinases, cellulases, cellobiases, and S. cerevisiae. Remaining arabinose and galacturonic acid were fermented with E. coli K011 to produce a peak ethanol yield of 0.299 g EtOH/g SBP. The methods approached and exceeded published results (0.277 g EtOH/g SBP) (Doran and Foster, 2000) to successfully increase ethanol yields. Ethanol concentrations were limited by high SBP moisture content and low solids loading rates.