| dc.contributor.author | Goswami, Kakolie | |
| dc.description.abstract | Fusarium head blight (FHB) is a fungal disease affecting cereal crops worldwide. FHB
involves multiple Fusarium species that create food safety concerns by producing
mycotoxins such as trichothecenes, zearalenone and moniliformin. Quantitative methods
allowing rapid risk assessment of mycotoxigenic Fusarium species are needed to detect
Fusarium spp. Real time quantitative PCR (qPCR) is a fast, sensitive and reliable
alternative to conventional culture methods. An objective of the study was to develop
TaqMan® based qPCR methods to detect, identify and quantify five different Fusarium
species associated with FHB, namely F. graminearum, F culmorum, F avenceum, F
poae and F sporotrichioides. The project was initiated with the selection of a protocol for
Fusarium DNA extraction. Three different commercially available DNA extraction kits,
including FastDNA ®, Qiagen® Blood & Tissue kit and Qiagen® DNeasy Plant kit were
evaluated for speed, DNA yield and quality. The results showed that FastDNA ® kit gave
the highest DNA yield in least time. TaqMan® Minor Groove Binder (MGB) probes for F
culmorum, F poae and F sporotrichioides were developed using qPCR primers from a
previous study. For F avenaceum and F. graminearum, TaqMan® based methods already
available were used with modified conditions for improved detection. The qPCR methods
were tested on a wheat system and the result was a qPCR system that rapidly identified
and quantified Fusarium species associated with FHB. | en_US |
| dc.publisher | North Dakota State University | en_US |
| dc.rights | NDSU policy 190.6.2 | en_US |
| dc.title | Development of Real-Time PCR Method for Detection, Identification and Quantification of Five Different Fusarium Species | en_US |
| dc.type | Thesis | en_US |
| dc.date.accessioned | 2024-03-08T21:18:32Z | |
| dc.date.available | 2024-03-08T21:18:32Z | |
| dc.date.issued | 2010 | |
| dc.identifier.uri | https://hdl.handle.net/10365/33727 | |
| dc.subject.lcsh | Fusarium -- Identification. | en_US |
| dc.subject.lcsh | Fusarium diseases of plants. | en_US |
| dc.subject.lcsh | Polymerase chain reaction -- Diagnostic use. | en_US |
| dc.rights.uri | https://www.ndsu.edu/fileadmin/policy/190.pdf | en_US |
| ndsu.degree | Master of Science (MS) | en_US |
| ndsu.college | Interdisciplinary Studies | en_US |
| ndsu.program | Food Safety | en_US |
| ndsu.advisor | Wolf-Hall, Charlene | |
