The advancements in medical operations and sciences have improved patient lives, but device-related infections and bacterial contamination remain significant concerns. Escherichia coli is a prominent bacterium causing various infections. Commonly used antibacterial materials in the health industry for surgical operations include silver, copper, zinc, titanium, and steel exhibit antibacterial properties due to their durability, corrosion resistance, and diverse applications. This study aimed to compare the antibacterial properties of titanium (Ti64), steel, and titanium coating on aluminum to inhibit the growth of Escherichia coli. The experiment utilized serial dilution and colony counting techniques to assess bacterial growth on the materials. Results showed that titanium has better antibacterial properties, with Ti64 coating on aluminum also displaying effectiveness but to a less extent. In contrast, steel was the least effective. The study highlights the need for further research to understand the underlying mechanisms of antibacterial behavior in these materials and their long-term efficacy in surgical operations, ultimately contributing to improved infection control in medical settings.