Concentrations of trace minerals within the body are known to impact reproductive processes. Thus, the current study analyzed the effects of using an injectable trace mineral supplement containing selenium, zinc, copper, and manganese during a superovulation protocol on embryo outcomes in donor beef cows and further effects on pregnancy rate in recipient females. We hypothesized that an injectable trace mineral (TM) supplement provided to cows fed to meet known nutrient requirements would increase TM status and influence superovulation, embryo characteristics, and enhance pregnancy rates. Our findings indicate that the injectable TM increased concentration of Se within the liver. However, superovulatory response, embryo production, quality grade, and developmental stage were not influenced by TM status. In addition, embryo treatment did not influence pregnancy rate, gestation length, or calf body weight.