| dc.contributor.author | Abeysekara, Nilwala Shyamen | |
| dc.description.abstract | Host-selective toxins, or necrotrophic effectors, are important determinants of
disease in both wheat-Stagonospora nodorum and wheat- Pyrenophora tritici-repentis
pathosystems. This study describes the identification, validation, and genomic analysis of
compatible host gene-effector interactions in these systems. In the wheat-S. nodorum
system, the Snn4-SnTox4 interaction was identified and validated using the hexaploid
wheat ( Tri ti cum aestivum L.) recombinant inbred populations 'Arina' x 'Forno' ( AF) and
Salamouni x 'Katepwa' (SK), respectively. The single dominant gene, Snn4, which
mapped to the short arm of chromosome 1 A in both populations, governs sensitivity to the
proteinaceous effector, SnTox4, which is estimated to be 10-30 kDa in size. The
compatible Snn4-SnTox4 interaction played a significant role in disease development in
both the AF and SK populations accounting for 41 % and 23.5% of the phenotypic
variation, respectively. Effects of the additional minor QTL were largely additive in both
genetic backgrounds. Molecular mapping in the SK population using microsatellites and
markers developed using bin-mapped expressed sequence tags (ESTs), and from ESTs
identified based on colinear studies with rice (Oryza sativa L.) and Brachypodium,
delineated Tsc2 to a 3.3 cM interval and confirmed its location on 2BS of hexaploid wheat.
The compatible T.~c2-Ptr ToxB interaction accounted for 54% of the disease variation in
the SK population. The marker XBE44454 l, which co-segregated with Tsc2 is diagnostic
of the gene and will be useful in marker-assisted selection (MAS). | en_US |
| dc.publisher | North Dakota State University | en_US |
| dc.rights | NDSU policy 190.6.2 | en_US |
| dc.title | Genomic Analysis of Tan Spot and Stagonospora Nodorum Blotch Resistance in Wheat | en_US |
| dc.type | Dissertation | en_US |
| dc.date.accessioned | 2023-12-27T21:26:22Z | |
| dc.date.available | 2023-12-27T21:26:22Z | |
| dc.date.issued | 2011 | |
| dc.identifier.uri | https://hdl.handle.net/10365/33473 | |
| dc.subject.lcsh | Wheat -- Diseases and pests -- Genetic aspects. | en_US |
| dc.subject.lcsh | Wheat -- Disease and pest resistance -- Genetic aspects. | en_US |
| dc.subject.lcsh | Stagonospora diseases. | en_US |
| dc.rights.uri | https://www.ndsu.edu/fileadmin/policy/190.pdf | en_US |
| ndsu.degree | Doctor of Philosophy (PhD) | en_US |
| ndsu.college | Interdisciplinary Studies | en_US |
| ndsu.department | Genomics, Phenomics, and Bioinformatics | en_US |
| ndsu.program | Genomics and Bioinformatics | en_US |
| ndsu.advisor | Faris, Justin D. | |
| ndsu.advisor | Friesen, Timothy L. | |
