Gylcoalkaloids (GA) are plant secondary metabolites that offer pests and disease resistance. Studies show correlation between GA content and CPB resistance. In this study, CPB resistance was assessed in a field trial at Grand Forks, ND, during 2012 for twenty-four genotypes from the NDSU Potato Breeding Program. Two treatments were applied, a block treated with imidacloprid (Admire®), and an untreated block. The treated block showed decreased CPB damage. Presence of aglycons (non-sugar moiety of GAs) was assessed by gas chromatography in foliar and tuber tissue. Distribution of GAs in the tuber was assessed to determine variation in tuber sections and whole tuber. Potato genotypes should be developed with tuber GAs levels below 20 mg/100 g fresh weight (FW) to ensure safety for human consumption. Focus should be on GAs that are only synthesized in the tuber, which will provide pests and disease resistance, while maintaining adequate yields and decreased inputs.