Focusing on various aspects of micropropagation with respect to well represented genotypes within Cercidiphyllum, this study is an attempt to broaden the experimental knowledge of the genus. The Tertiary relict Cercidiphyllum (Cercidiphyllaceae) is endemic to Japan and China and consists of two deciduous tree species, common katsura, C. japonicum Sieb. & Zucc. and the hiro-ha-katsura, C. magnificum Nakai. To date, there exists no literature on the in vitro requirements of C. magnificum and limited information on C. japonicum. Prized as specimen trees, the importance of Cercidiphyllum extends beyond its ornamental merit and regarded a multifaceted genetic resource. Subsequent protocols and analysis will not only be useful for nursery production and cultivar improvement but aid in ongoing conservation efforts of this rare genus.