Releasing bread wheat (Triticum aestivum) cultivars with resistance to Fusarium head blight (FHB) disease can be endangered by narrowing the variation in genetic sources. However, resistance to FHB rely on five different types. FHB resistance types I, II, III, IV and V were assessed in field and greenhouse under multiple locations and years experiment’s combination. In our study, the genetic of FHB resistance in two widely cultivated hard red spring wheat varieties (‘Glenn’ and ‘Parshall’) were dissected. The specific objectives of the study were to generate recombinant inbred lines (RIL) populations, phenotypic assessment for FHB resistance, different informative genotypic marker data, genetic map, and finally QTL analysis. For Glenn/MN00216-4 (GM) population, 112 RIL were developed; while for Parshall/Reeder (PR) population, 110 RIL were developed. The RIL, checks and the two parents were evaluated for five FHBrelated and one agronomic-related traits over two to six environments in North Dakota, Minnesota, and South Dakota. Two genetic maps were developed covering 2,229 cM of length using 645 DArT markers for GM population, and 470.4 cM length using 154 DArT/SNP combined markers for PR population. Composite interval mapping identified 37 QTL for the GM population, and 10 QTL for the PR population. Results showed that Glenn lacks the major consistent (Fhb1 and Fhb5A) QTL from the Chinese source Sumai3, while acquired (Fhb2). Parshall proved to be domestic with no exotic resistance background, though it acquired similar genomic regions to Fhb2 of Sumai3. PR genome contains five major QTL including three novel QTL with multiple FHB resistance and two with stable effect (1AS and 4BL) across at least two environments. Along with these previously identified QTL for FHB resistance, in both populations, new QTL were also identified such as Fhb-1B1L.c and 7D1S.b in Glenn, and Fhb.5AL, 7AS and 4BL in Parshall. In conclusion, our study added to the wheat genome, two genetic maps, new QTL for FHB resistance and two germplasms with new recombination for QTL and/or resistance sources. Finally, Glenn and Parshall can be of great importance if implemented in wheat enhancement and molecular assisted breeding programs nationally and internationally.