Marker Assisted Backcross Selection for Virus Resistance in Pea (Pisum Sativum L.)
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Viruses are destructive plant pathogens, which cause significant yield loss and reduced grain quality. Pea Seed-borne Mosaic Virus (PSbMV) is an economically important viral disease in pea (Pisum sativum L.) and recently detected in the Northern Great Plains (NGP) in 2012. PSbMV is aphid-transmitted from plant to plant and can be seed-borne. It causes malformed leaves, discolored or split seed, and reduced size and number of seeds. Host resistance to PSbMV-P4 is conferred by a recessive gene, sbm-1. Marker assisted backcross breeding using the 4Egenomic primers previously developed assisted in transferring the single resistance allele located on LG VI from ‘Lifter’ into locally adapted breeding lines. After two backcrosses and allowing plants to self-pollinate to the B2F2, individuals were inoculated with PSbMV-P4 isolate to validate resistance. The BC2F3 populations were tested in a field evaluation trial for disease resistance against the PSbMV-P4 strain in the NGP and for agronomic adaptation.