Grain protein content is an essential component to producing a profitable Hard Red Spring Wheat (HRSW) (Triticum aestivum L.) crop in the northern Great Plains. Growers can increase grain protein content through in-season N fertilization; however, the cost of these applications may outweigh the benefits. Predicting the grain protein content of early-season HRSW would give growers crucial information as they decide whether to apply in-season fertilizer to boost grain protein content. This research encompasses three studies; two of which aim to predict grain protein content with hand-held and aerial sensors respectively, and a third, which investigates the optimal rate, timing, and source of N fertilizer to boost grain protein content. Results of these experiments seemed to be greatly influenced by environmental factors. Findings of this research suggest that an in-season N application should be used for ameliorative purposes and not as a regular practice.