| dc.contributor.author | Mazaheri, Lucy | |
| dc.description.abstract | In common bean (Phaseolus vulgaris), the main seed storage pests are the bruchid beetles. Damage done to the seed by the larvae has a large impact on seed quality and yield. Arcelin (ARC), phytohaemagglutinin (PHA), and α-amylase inhibitor (α-AI) are linked seed storage proteins that form the APA locus on chromosome Pv04 and are associated with resistance. A major breeding objective is to introduce bruchid resistance into common bean from a resistant tepary genotype, G40199, by introgressing the resistant APA locus into susceptible common bean backgrounds. Here we developed a molecular marker that tracks the introgression. A set of PCR primers to the α-amylase inhibitor locus amplified a DNA fragment that showed a 45 base pair insertion in the middle of a lectin Leg_b domain. This enhanced locus characterization and insertion/deletion marker may preclude the need for bruchid resistance screening early in the breeding. | en_US |
| dc.publisher | North Dakota State University | en_US |
| dc.rights | NDSU policy 190.6.2 | |
| dc.title | Development of a Molecular Marker to Track APA G40199 Introgression in Common Bean for Bruchid Resistance | en_US |
| dc.type | Thesis | en_US |
| dc.date.accessioned | 2019-02-15T22:00:07Z | |
| dc.date.available | 2019-02-15T22:00:07Z | |
| dc.date.issued | 2018 | en_US |
| dc.identifier.uri | https://hdl.handle.net/10365/29300 | |
| dc.subject.lcsh | Common bean -- Seeds -- Diseases and pests. | |
| dc.subject.lcsh | Bruchidae -- Control. | |
| dc.subject.lcsh | Common bean -- Disease and pest resistance -- Genetic aspects. | |
| dc.description.sponsorship | United States. Agency for International Development | en_US |
| dc.description.sponsorship | United States. Global Hunger and Food Security Initiative (Cooperative Agreement No. EDH-A-00-07-00005-00) | en_US |
| dc.rights.uri | https://www.ndsu.edu/fileadmin/policy/190.pdf | |
| ndsu.degree | Master of Science (MS) | en_US |
| ndsu.college | Agriculture, Food Systems and Natural Resources | en_US |
| ndsu.department | Plant Sciences | en_US |
| ndsu.program | Genomics and Bioinformatics | en_US |
| ndsu.advisor | McClean, Phillip E. | |
