Bean rust (Uromyces appendiculatus (Pers) Unger), is a highly virulent pathogen that negatively affects bean (Phaseolus vulgaris L.) seed yield. Over time, bean rust has the capacity to generate new races that overcome the existing resistance in cultivars. An alternative to control plant diseases is through breeding and pyramiding resistance genes. However, these genes need to be characterized first, to be used effectively. An F2 population from the cross UI-114 /Mexico 309 (Ur-5 gene) was evaluated with race 20-3 being prevalent in ND. A set of InDel markers were designed and tested for potential use in MAS. It was confirmed that the Ur-5 is a single dominant gene and it was located between 547.7-1,396.4 Kb in the Pv04. The candidate genes found are mostly NB-ARC and LRR domains, and it included some genes models that encode cytochrome P450, and protein kinases that are also related to disease resistance.