| dc.contributor.author | Kim, Changhyeon | |
| dc.description.abstract | An Agrobacterium-mediated transformation system of purple raspberry ‘Amethyst’ was established after a series of experiments that determined the effect of genotype, inoculum density, and co-cultivation time on transformation. In this study, a plant regeneration protocol was established for ‘Joan J’ and ‘Polana’ (the regeneration protocol of ‘Amethyst’ was previously developed). Agrobacterium-mediated transformation was conducted for all three cultivars. The minimum killing level of hygromycin B and kanamycin was determined. Inoculum density and co-cultivation time were optimized. Polymerase chain reaction (PCR) verified a successful transformation of ‘Amethyst’ with the frequency of 3.3 ~ 4.4 % when leaves were infected with Agrobacterium EHA105 at the cell density of OD600 0.3 and co-cultivated for 3 days in the medium with 25.0 mg∙l-1 kanamycin. Transgenic lines with the PtFIT gene were hydroponically grown under iron sufficiency or deficiency. The real-time quantitative PCR verified the gene expression in response to iron sufficiency and deficiency conditions. | en_US |
| dc.publisher | North Dakota State University | en_US |
| dc.rights | NDSU policy 190.6.2 | |
| dc.title | Development of a Genetic Transformation System of Raspberry Cultivars for Gene Function Analysis | en_US |
| dc.type | Thesis | en_US |
| dc.date.accessioned | 2019-01-18T21:44:54Z | |
| dc.date.available | 2019-01-18T21:44:54Z | |
| dc.date.issued | 2018 | en_US |
| dc.identifier.uri | https://hdl.handle.net/10365/29223 | |
| dc.subject.lcsh | Raspberries -- Genetic engineering. | |
| dc.subject.lcsh | Plant genetic transformation. | |
| dc.subject.lcsh | Agrobacterium. | |
| dc.identifier.orcid | 0000-0001-8631-2774 | |
| dc.rights.uri | https://www.ndsu.edu/fileadmin/policy/190.pdf | |
| ndsu.degree | Master of Science (MS) | en_US |
| ndsu.college | Agriculture, Food Systems and Natural Resources | en_US |
| ndsu.department | Plant Sciences | en_US |
| ndsu.program | Plant Sciences | en_US |
| ndsu.advisor | Dai, Wenhao | |
